WebThe decalcified paraffin-embedded sections were prepared and stained with HE and Safranin O/fast green for cartilage assessment (Mankin score), staging, and bone histomorphometry; the pathologic features of the cartilage and … WebFeb 3, 2024 · OARSI score analysis was used to confirm the protective characteristics of MA (Figure 5(b)). Safranin O staining was markedly increased in the MA-treated group when compared with the OA group; hematoxylin-eosin staining supported the outcomes of safranin O and showed that MA is able to inhibit the hyperplasia of synovium.
Safranin-O Staining Solution Safranin-O is often used to identify ...
WebSafranin-O staining (SafO) (0.2%) and counterstaining with Fast Green (0.2%) ... Combined with the results of HE staining, Mankin score grading was performed (Figure 5B, bottom panel, and ... SafO staining showed cartilage surface defects in AggH-Tam group mice classified as early-stage OA while no obvious changes were observed in the other ... WebIHC analysis indicated that expression of collagen II, a specific marker of hyaline cartilage, was similar in the Fig. 4 Histological analysis. a H&E staining. b Safranin O/fast green staining. H&E and safranin O/fast green staining showed that the normal group and iMSC-Exos group presented typical hyaline features with a smooth cartilage ... hisinoneexternkarlsruhe
Pathological, Morphometric and Correlation Analysis of the …
WebApr 20, 2024 · The intensity of safranin O staining, as assessed by image analysis, which is proportional to the glycosaminoglycan content of cartilage, showed marked differences ... Many publications detail the use of the Mankin Score for grading human cartilage pathology. The original Mankin Score (or HHGS score) ranges from 0 (normal) to 14 ... WebOct 1, 2010 · Both Safranin O and Toluidine blue are cationic dyes that stain proteoglycans as well as glycosaminoglycans. Toluidine blue (Table II) has been reported to provide … WebIn order to assess the changes in tissue composition, in-depth analysis of the histomorphometric articular cartilage of the femur and tibia was performed as described in Materials and Methods, including calculation of the modified Mankin score, and assessment of R tm and CC thick by Safranin-O staining at central regions of the femur and tibia in … his in japanese