Pherd30t
Web1. dec 2024 · We describe here the use of pHERD30T plasmid, a P BAD -based shuttle vector carrying a gentamicin resistance marker [. [9] ]. This plasmid was selected because it is a … Web13. apr 2024 · 将pherd30t及重组质粒pherd30t-ypjd转化入营养缺陷菌株e.coliwm3064，并通过接合的方式进入宿主工程菌株pv-4δypjd中，空载pherd30t转化入营养缺陷菌 …
Pherd30t
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Web10. mar 2024 · sites of pHERD30T. For experiments in which plasmid-borne crRNAs were used, the resulting plasmids were introduced into PAO1 tn7::LbCas12a directly via … Web5. sep 2024 · Lysates were plated on lawns of PA14 expressing a crRNA targeting phage JBD44 from pHERD30T to isolate phages carrying and expressing the anti-CRISPR locus. …
Web1. jan 2024 · Transform 50 ng of pHERD30T and pHERD30T-targ in commercially available chemically competent E. coli DH5α (subcloning efficiency) as per manufacturer's instructions.-Add 950 µL of LB to the transformation mix, incubate at 37 ˚C for 1h at 180 rpm.-Plate 50 µL on LB agar supplemented with 30 µg/mL of gentamicin sulfate- WebThe recombinant pHERD30T_GFP(ASV) vector. The recombinant pHERD30T vector, encoding the GFP(ASV) protein was obtained by cloning the gfp(ASV) gene into the original vector using the In-Fusion HD Cloning Plus kit; the vector map was designed with the software SNAPGENE v. 4.2.6.
WebIn this work we present the rapid synthesis of nanocrystals of a fluorescent microporous metal-organic framework using an ultrasonic method, and their utility for selective … WebPlasmid pCas3cRh from Dr. Joseph Bondy-Denomy's lab contains the inserts RhaR, RhaS, pRhaBAD, I-C crRNA, Cas3, Cas5, Cas8, and Cas7 and is published in Nat Methods. 2024 …
Web5. jún 2024 · We were successful in removing selectable marker genes in transgenic B. napus plants using all three co-transformation systems developed in this study. It was …
WebEscherichia-Pseudomonas shuttle vector pHERD30T: eukaryotic vectors: Activation tagging vector pGA2772: Activation tagging vector pSKI074: Adenoviral expression vector Ad … forklift speed limit oshaWeb23. máj 2024 · To express rhlR in the ΔQS-Eind strain, the rhlR coding region was cloned under the control of the l-arabinose-controlled P BAD promoter in the pHERD30T vector . … forklift speed limit in singaporehttp://www.xjishu.com/zhuanli/27/202410072760.html difference between judger and perceiverWebNational Center for Biotechnology Information forklift speed controllerWeb23. jan 2015 · 噬菌体内溶素基因分离、鉴定及功能研究.pdf. 噬菌体内溶素基因分离、鉴定及功能研究,噬菌体,t4噬菌体,噬菌体展示,t2噬菌体,噬菌体展示技术,温和噬菌体,烈性噬菌体, … difference between judges and justicesWebtechnology into the pHERD30T vector amplified with primers pHERD30T_forward and pHERD30T_reverse (table S1). Clinical strains were transformed as previously described [18]. Redox sensitivity assay Strains were grown o. n. in 50% LB medium containing 0% or 2% MIC of azithromycin. Cultures were then mixed with molten 0.5% difference between judge and refereeWeb7. jún 2024 · Each donor strain carried unmodified pHERD30T or a derivative encoding the complementary protospacer sequence on the leading strand (LDS) or lagging strand (LGS) with or without a PAM (Supplementary Figure S3). The strains were grown overnight in LB broth supplemented with the appropriate antibiotics and CRISPR-Cas inducers. Donor and … forklift speed limit alarm