Ctll cell
Webctll-2 Cells Products Every step of the way, a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research results. Web- optimized for applications with CTLL-2 cells Catalog Number: WST1.CTLL150813.1/.2/.3 4. Assay Protocol Tips before starting: Working concentration of WST-1 ready-to-use cell proliferation Kit Add 10 µl/well of WST-1 reagent from WST-1 CTLL-2 cell proliferation solution (ready-to-use) to CTLL-2 cells cultured in 100 µl/well culture medium.
Ctll cell
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WebAug 10, 2024 · a The activity of the indicated proteins was detected by a CTLL-2 proliferation assay.b Balb/c mice (n = 5/group) were inoculated with 3 × 10 6 A20 cells. … WebApr 14, 2024 · CD4+ T cells are typically considered as ‘helper’ or ‘regulatory’ populations that support and orchestrate the responses of other lymphocytes. However, they can also develop potent granzyme (Gzm)-mediated cytotoxic activity and CD4+ cytotoxic T cells (CTLs) have been amply documented both in humans and in mice, particularly in the …
WebApr 11, 2024 · Credit: Medical gallery of Blausen Medical 2014, WikiJournal of Medicine. An international research team has discovered a new cell state in embryonic airway … WebNov 1, 2002 · In order to compare functions of IL-2 and IL-15 on cellular proliferation and survival, we used the murine cytokine-dependent CTLL-2 cell line. Both cytokines …
WebThe cells require IL-2 for growth and are used to assay for its presence in conditioned media. 애플리케이션 CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. WebInterleukin-15 (IL-15) is a pleiotropic cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). This glycoprotein is a member of the 4 α-helix-bundle family of cytokines and is required for the differentiation of effector CD8 + and memory phenotype CD8 + T cells (3,4).
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http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf draw a box in adobe acrobatWebCutaneous T-cell lymphoma (CTCL) is a rare type of blood cancer. It begins in a type of white blood cell called the T-lymphocyte (T-cell). T-cells help prevent infections and other diseases. As odd as it sounds, most T-cells … employee discount formWebProtocol: Seed CTLL-2 cells at a concentration of 4 × 10 3 cells/well in 100 μL culture medium containing various amounts of IL-2 (final concentration e.g., 0.005 – 25 ng/mL) … draw a box in blenderWebJan 9, 2024 · CTLL-2 cells purchased from ATCC were cultured in RPMI complete with 10% T-STIM culture supplement with ConA (Corning). Twenty-four hours before signalling studies, CTLL-2 cells were resuspended ... employee discount for mahindra resortsWebCTLL-2 cells were incubated with serial dilutions of an international standard of IL-15 and IL-15 obtained from E. coli initially diluted to 10 3 and in presence of the neutralizing monoclonal... draw a box in excelWebJan 19, 2010 · This approach is reasonable as CTLL-2 cells did not respond to endogenous amounts of cytokines present in the normal mouse serum and, more importantly, displayed a comparable sensitivity to IL-2 and both forms of IL-2/mAb complexes ( Fig. S2 ). draw a box in 3dWebJun 9, 2024 · The CTLL-2 cell line was a gift from Prof. Zhigang Tian. CTLL-2 cells were cultured in RPMI medium modified (HyClone, Logan, UT) supplemented with 10% FBS (Biological Industries, Beit HaEmek, Israel), 200 IU/mL rhIL-2, 100 U/mL penicillin and 100 µg/mL streptomycin (Sangon Biotech, Shanghai, China) at 37 °C in a 5% CO 2 incubator. draw a box in adobe